Asilomar — Crocker HallDear 17th IHIWS Participants,

Thank you for attending the 17th International HLA & Immunogenetics Workshop and Conference. The 17th IHIW NGS HLA data is now available, as well as the tools applications used in the Workshop.

If you would like an archive of the 17th International Histocompatibility and Immunogenetics Workshop and Workshop Conference event, you can view a copy of the 17th IHIWS Schedule and Program or read the newsletter.

17th IHIWS Organizing Committee

Components & Projects

The 17th IHIWS will studied HLA and KIR genomics, defined full length MHC sequences, mapped serologic epitopes and developed robust informatics for HLA and KIR research through the use of NGS, single antigen beads (SAB) and flow cytometry.

The 17th IHIWS projects fell under the following components:

(Click on the component name for more information)

All Serologic Epitopes Projects All NGS of Full-length HLA Genes Projects NGS of Full-length KIR Genes Mapping of Serologic Epitopes Hematopoietic Cell Transplantation (HCT) Quality Control & Quality Assurance Informatics of Genomic Data NGS of Full-length HLA genes

Latest Developments (Read All)

NGS of Full-length HLA genes NGS of Full-length HLA genes -

The HLA genes are the most polymorphic loci in the human genome; over 12,000 genetic variants (alleles) have been identified at 19 HLA genes, with some individual genes displaying several thousand alleles. The allelic and structural variation that characterizes these genes pose extreme challenges for routine genotyping of these genetic loci; this variation cannot be characterized by a few SNPs, and the high level of polymorphism confounds de novo sequence assembly efforts. Until recently, HLA genotyping has been accomplished using a variety of PCR-based approaches involving sequence-specific oligonucleotide probe (SSOP) hybridization and/or Sanger sequencing of 400-600 base pair amplicons. These... Read More

Component: NGS of full length HLA genes: Preliminary results of the Pilot Study -

Lisa E Creary1, Steven J Mack2 and Marcelo Fernandez-Vina1
1Department of Pathology, Stanford Blood Center
2Children’s Hospital Oakland Research Institute


The ultimate goal of the 17th International HLA and Immunogenetics Workshop (IHIW) is to advance the fields of Histocompatibility and Immunogenetics (H & I) research through the application of Next-Generation Sequencing (NGS) technologies for HLA and KIR genotyping, and to advance the development of NGS technologies tailored to meet the needs of the H & I community.

In 2014, we initiated an international multi-center pilot study in order to assess the performance of various NGS protocols, platforms,... Read More

T Cell Receptor (TCR)-HLA Interaction -

The T cell receptor is the natural receptor of the HLA-peptide complex and interacts directly with MHC molecules, notably their CDR regions.  The goal of this project will be to combine analysis of TCR and HLA in the context of disease, vaccination/immune alterations and populations.  We would expect samples to be CD4 or CD8 T cells and to have full HLA class I and class II typing by NGS.

Required NGS HLA loci: HLA-A, B, C, DRB1, DRB3/4/5, DQB, HLA-DQA, DPA, DPB
Optional NGS HLA Loci: MICA, MICB

Subprojects for this component will include:
  • Disease study:  This will involve... Read More
  • Extension of HLA allele sequences by full-length HLA allele-specific hemizygous Sanger sequencing (SSBT) -

    The Human Leucocyte Antigen (HLA) is one of the most polymorphic gene systems present in the human genome and due to this high polymorphism, the golden standard for typing at the allele level has been and still is sequence based typing. Since sequencing strategies have mainly focused on identification of the peptide binding groove, therefore the majority of HLA alleles lack full length sequence information.

    The goal of this project is to extend the sequences of as many incompletely covered HLA alleles as possible by full length unambiguous Sanger Sequencing. Although NGS approaches are currently implemented in several laboratories, many... Read More

    Recent Events

    database vendor summit

    17th IHIWS Vendor Summit
    SEPTEMBER 25, 2016

    During this Summit, we had presentations from Kazutoyo Osoegawa PhD, Stanford HIDPL, who described the Data entry experience from various participants into the IHIWS database, demonstrating the need for standarized reporting.

    We also heard from Steve Mack PhD, CHORI, whose presentation provided an overview of the workshop and specifically the database, highlighting the need to have uniform Data content and why all NGS data and meta-data should be compatible and comparableallowing all formats to be equally interpretable during analysis. Steve described the concept of Minimum Information for Reporting Immunogenomic NGS Genotyping (MIRING) and why it is essential to analysis to be performed on the data collected.

    Last, we heard from Bob Milius PhD, Bioinformatics Research NMDP®/Be The Match®. Bob presentation focused on MIRING & HML reporting as well as XML schemas. In a second presentation, Bob described Genome Sequence Coordinates, advantages and disadvantages of One-Based Vs. Zero-Based systems and matching SequenceBlock to the reference sequence.

    Read more / View the presentations